Claire CHAZAUD

Resume

Claire CHAZAUDprofile picture

Present: PI of the team: Molecular mechanisms of cell lineage differentiation in the preimplantation embryo.

2012: DR2 INSERM, PI at the GReD

2005 : CR1 INSERM, PI at the GReD

2003 to present: PI of team n°5 at the GReD (Clermont-Fd)

2002: Recipient of an "AVENIR" grant.

February 1999 to June 2003: post-doctoral fellow in Dr. Janet RossantLaboratory (Toronto, Canada). Research topics: 1- Cell lineage specification in the preimplantation mouse embryo. 2-Involvement of the Wnt/beta-Catenin pathway during A-P axis formation in the mouse.

1994-1998: PhD Université L. Pasteur,IGBMC (Strasbourg), director Dr. P. Dollé. Research topic: Characterisation of RA inducible genes expressed during  mouse development (supervisors Dr. P. Dollé and Prof. P. Chambon).

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Research
Publications

Research

We are looking for a Post-Doc!

Our team is interested in deciphering the molecular mechanisms regulating one of the first cell differentiation event that takes place in the embryo: the binary specification of epiblast (Epi) and Primitive Endoderm (PrE) within the Inner Cell Mass of the blastocyst during preimplantation.

We have recently described the role of Nanog (Frankenberg et al, 2011 Dev Cell) and Gata6 (Bessonnard et al, 2014 Development) and shown that the balance between these two transcription factors determines the number and the timing of Epi and PrE cell specification.

In collaboration with the team of A. Goldbeter/ G. Dupont a mathematical model was built (Bessonnard et al, 2014 Development) , uncovering novel features that were are currently investigating.

Publications

  • 2022
  • 2021
    • D. Meistermann, A. Bruneau, S. Loubersac, A. Reignier, J. Firmin, V. Francois-Campion, S. Kilens, Y. Lelievre, J. Lammers, M. Feyeux, P. Hulin, S. Nedellec, B. Bretin, G. Castel, N. Allegre, S. Covin, A. Bihouee, M. Soumillon, T. Mikkelsen, P. Barriere, C. Chazaud, J. Chappell, V. Pasque, J. Bourdon, T. Freour and L. David, “Integrated pseudotime analysis of human pre-implantation embryo single-cell transcriptomes reveals the dynamics of lineage specification.”, Cell stem cell, vol. 28 (9) , pp. 1625–1640.e6, 2021.
    • K. Boutourlinsky, N. Allegre and C. Chazaud, “Ex Vivo Culture for Preimplantation Mouse Embryo to Analyze Pluripotency.”, Meth. Mol. Biol., vol. 2214 , pp. 1–10, 2021.
  • 2020
    • A. Huyghe, G. Furlan, D. Ozmadenci, C. Galonska, J. Charlton, X. Gaume, N. Combemorel, C. Riemenschneider, N. Allegre, J. Zhang, P. Wajda, N. Rama, P. Vieugue, I. Durand, M. Brevet, N. Gadot, T. Imhof, B. Merrill, M. Koch, P. Mehlen, C. Chazaud, A. Meissner and F. Lavial, “Netrin-1 promotes naive pluripotency through Neo1 and Unc5b co-regulation of Wnt and MAPK signalling.”, Nature cell biology, vol. 22 (4) , pp. 389–400, 2020.
  • 2019
    • T. Azami, C. Bassalert, N. Allegre, L. Valverde Estrella, P. Pouchin, M. Ema and C. Chazaud, “Regulation of the ERK signalling pathway in the developing mouse blastocyst.”, Development, vol. 146 (14) , 2019.
    • A. Tosenberger, D. Gonze, C. Chazaud and G. Dupont, “Computational models for the dynamics of early mouse embryogenesis.”, The International journal of developmental biology, vol. 63 (3-4-5) , pp. 131–142, 2019.
  • 2018
  • 2017
    • A. Tosenberger, D. Gonze, S. Bessonnard, M. Cohen-Tannoudji, C. Chazaud and G. Dupont, “A multiscale model of early cell lineage specification including cell division.”, NPJ systems biology and applications, vol. 3 , pp. 16, 2017.
  • 2016
    • A. Fiorenzano, E. Pascale, C. D'Aniello, D. Acampora, C. Bassalert, F. Russo, G. Andolfi, M. Biffoni, F. Francescangeli, A. Zeuner, C. Angelini, C. Chazaud, E. Patriarca, A. Fico and G. Minchiotti, “Cripto is essential to capture mouse epiblast stem cell and human embryonic stem cell pluripotency.”, Nature communications, vol. 7 , pp. 12589, 2016.
    • C. Chazaud and Y. Yamanaka, “Lineage specification in the mouse preimplantation embryo.”, Development, vol. 143 (7) , pp. 1063–74, 2016.
    • L. De Mot, D. Gonze, S. Bessonnard, C. Chazaud, A. Goldbeter and G. Dupont, “Cell Fate Specification Based on Tristability in the Inner Cell Mass of Mouse Blastocysts.”, Biophysical journal, vol. 110 (3) , pp. 710–22, 2016.
  • 2015
    • S. Chauveau and C. Chazaud, “Assessment of cell lineages and cell death in blastocysts by immunostaining.”, Meth. Mol. Biol., vol. 1222 , pp. 175–80, 2015.
  • 2014
    • S. Hermitte and C. Chazaud, “Primitive endoderm differentiation: from specification to epithelium formation.”, Philosophical transactions of the Royal Society of London. Series B, Biological sciences, vol. 369 (1657) , 2014.
    • S. Bessonnard, L. De Mot, D. Gonze, M. Barriol, C. Dennis, A. Goldbeter, G. Dupont and C. Chazaud, “Gata6, Nanog and Erk signaling control cell fate in the inner cell mass through a tristable regulatory network.”, Development, vol. 141 (19) , pp. 3637–48, 2014.
    • J. Artus and C. Chazaud, “A close look at the mammalian blastocyst: epiblast and primitive endoderm formation.”, Cell. Mol. Life Sci., vol. 71 (17) , pp. 3327–38, 2014.
  • 2013
  • 2012
    • F. Lavial, S. Bessonnard, Y. Ohnishi, A. Tsumura, A. Chandrashekran, M. Fenwick, R. Tomaz, H. Hosokawa, T. Nakayama, I. Chambers, T. Hiiragi, C. Chazaud and V. Azuara, “Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development.”, Genes Dev., vol. 26 (13) , pp. 1445–58, 2012.
  • 2011
    • S. Frankenberg, F. Gerbe, S. Bessonnard, C. Belville, P. Pouchin, O. Bardot and C. Chazaud, “Primitive endoderm differentiates via a three-step mechanism involving Nanog and RTK signaling.”, Dev. Cell, vol. 21 (6) , pp. 1005–13, 2011.
    • H. Elatmani, V. Dormoy-Raclet, P. Dubus, F. Dautry, C. Chazaud and H. Jacquemin-Sablon, “The RNA-binding protein Unr prevents mouse embryonic stem cells differentiation toward the primitive endoderm lineage.”, Stem cells (Dayton, Ohio), vol. 29 (10) , pp. 1504–16, 2011.
  • 2008
    • C. Chazaud, “[Early embryogenesis in mammals: stem cells and first commitment steps].”, Med Sci (Paris), vol. 24 (12) , pp. 1043–8, 2008.
    • F. Gerbe, B. Cox, J. Rossant and C. Chazaud, “Dynamic expression of Lrp2 pathway members reveals progressive epithelial differentiation of primitive endoderm in mouse blastocyst.”, Dev. Biol. (NY), vol. 313 (2) , pp. 594–602, 2008-01-15 2008.
  • 2006
    • C. Chazaud and J. Rossant, “Disruption of early proximodistal patterning and AVE formation in Apc mutants.”, Development, vol. 133 (17) , pp. 3379–87, 2006.
    • C. Chazaud, Y. Yamanaka, T. Pawson and J. Rossant, “Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway.”, Dev. Cell, vol. 10 (5) , pp. 615–24, 2006.
  • 2003
    • J. Rossant, C. Chazaud and Y. Yamanaka, “Lineage allocation and asymmetries in the early mouse embryo.”, Philosophical transactions of the Royal Society of London. Series B, Biological sciences, vol. 358 (1436) , pp. 1341–8; discussion 1349, 2003.