Fabrice SAEZ
PhD in Reproductive Biology
Resume
1997-2000 : Thèse en Biologie de la Reproduction au CECOS de l'Université d'Auvergne.
2001-2003 : Post-doctorat au sein de l'équipe du Dr Robert SULLIVAN à l'Université LAVAL à Québec (QC, Canada)
Depuis septembre 2003 : Maître de conférences en physiologie animale (section 66) à l'Université Blaise Pascal devenue l'Université Clermont Auvergne. HDR en 2015.
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Research
Au sein de l’équipe MePTI (Mechanisms of Post-Testicular Infertility), les activités de recherche sont centrées sur la maturation épididymaire des gamètes mâles. À l’aide de modèles murins génétiquement modifiés pour inhiber la fonction d'enzymes antioxydantes post-testiculaires, nous étudions l'impact du stress oxydant épididymaire sur le patrimoine génétique et épigénétique des gamètes mâles, principalement leurs ARN non codants et les marques de méthylation de l'ADN.
Un deuxième axe de recherche est centré sur les mécanismes présents au sein de l’épididyme qui lui permettent de répondre au défi immunitaire représenté par la protection des spermatozoïdes contre une réaction auto-immune tout en protégeant l’organe et les gamètes des micro-organismes infectieux ascendants.
Enfin, dans le cadre d'un projet à vocation appliquée, nous développons un programme de recherche pour la mise au point d'un vaccin immunocontraceptif en vue d'endiguer les proliférations cycliques d'un rongeur sauvage nuisible, le rat taupier, Arvicola terrestris Scherman (projet CONTRACAMP).
Ces thèmes de recherche sont abordés à l’aide de différentes techniques et technologies : études biologiques classiques des gamètes mâles (motilité, morphologie, viabilité, réaction de l’acrosome, fragmentation de l’ADN via des analyses sur une plateforme semi-automatisée), niveau d’oxydation de l’ADN par immunofluorescence et microscopie confocale, immunoprécipitation de l'ADN oxydé (Oxidip) et séquençage nouvelle génération, cytométrie en flux et tri cellulaire, biologie moléculaire (qRT-PCR, western-blot…).
Publications
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2023
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“Spermatozoa isolation with Felix TM outperforms conventional density gradient centrifugation preparation in selecting cells with low DNA damage.”, Andrology, 2023.
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2021
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“When idiopathic male infertility is rooted in maternal malnutrition during the perinatal period in mice.”, Biol Reprod, 2021.
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“Field study reveals morphological and neuroendocrine correlates of seasonal breeding in female water voles, Arvicola terrestris.”, General and comparative endocrinology, vol. 311 , pp. 113853, 2021.
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“Photoperiod is involved in the regulation of seasonal breeding in male water voles (Arvicola terrestris).”, J. Exp. Biol., 2021.
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“Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes”, International Journal of Molecular Sciences, vol. 22 (18) , pp. 9965, 2021.
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2020
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“Differential expression and localisation of TGF-beta isoforms and receptors in the murine epididymis.”, Scientific reports, vol. 10 (1) , pp. 995, 2020.
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2019
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“Of vessels and cells: the spatial organization of the epididymal immune system.”, Andrology, vol. 7 (5) , pp. 712–718, 2019.
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“Impairment of sperm maturation and capacitation due to diet-dependent cholesterol overload.”, Andrology, vol. 7 (5) , pp. 654–661, 2019.
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“The epididymal immune balance: a key to preserving male fertility.”, Asian J. Androl., 2019.
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“Dietary Cholesterol and Lipid Overload: Impact on Male Fertility.”, Oxidative medicine and cellular longevity, vol. 2019 , pp. 4521786, 2019.
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2018
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“Nuclear Integrity but Not Topology of Mouse Sperm Chromosome is Affected by Oxidative DNA Damage.”, Genes, vol. 9 (10) , 2018.
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“Slc26a3 deficiency is associated with epididymis dysplasia and impaired sperm fertilization potential in the mouse.”, Molecular reproduction and development, vol. 85 (8-9) , pp. 682–695, 2018.
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“Dual extraction of mRNA and lipids from a single biological sample.”, Scientific reports, vol. 8 (1) , pp. 7019, 2018.
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“Comprehensive overview of murine epididymal mononuclear phagocytes and lymphocytes: Unexpected populations arise.”, Journal of reproductive immunology, vol. 126 , pp. 11–17, 2018.
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2017
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“Dyslipidemia alters sperm maturation and capacitation in LXR-null mice.”, Reproduction, 2017.
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2016
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“Liver X Receptors (LXRs) Alpha and Beta Play Distinct Roles in the Mouse Epididymis.”, Biol Reprod, vol. 94 (3) , pp. 55, 2016.
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2015
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“Oxidative DNA damage in mouse sperm chromosomes: Size matters.”, Free Radic. Biol. Med., vol. 89 , pp. 993–1002, 2015.
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“Posttesticular sperm maturation, infertility, and hypercholesterolemia.”, Asian J. Androl., vol. 17 (5) , pp. 742–8, 2015.
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2013
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“DNA oxidative damage in mammalian spermatozoa: where and why is the male nucleus affected?”, Free Radic. Biol. Med., vol. 65 , pp. 719–23, 2013.
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“Indoleamine 2,3-dioxygenase 1 (ido1) is involved in the control of mouse caput epididymis immune environment.”, PLoS ONE, vol. 8 (6) , pp. e66494, 2013.
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2012
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“Epididymis response partly compensates for spermatozoa oxidative defects in snGPx4 and GPx5 double mutant mice.”, PLoS ONE, vol. 7 (6) , pp. e38565, 2012.
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2011
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“Glutathione peroxidases at work on epididymal spermatozoa: an example of the dual effect of reactive oxygen species on mammalian male fertilizing ability.”, J. Androl., vol. 32 (6) , pp. 641–50, 2011.
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“Liver X receptors, lipids and their reproductive secrets in the male.”, Biochim. Biophys. Acta, vol. 1812 (8) , pp. 974–81, 2011.
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“Dietary cholesterol-induced post-testicular infertility.”, PLoS ONE, vol. 6 (11) , pp. e26966, 2011.
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2010
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“Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state.”, J. biol. chem., vol. 286 (10) , pp. 8030–42, 2010.
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“Epididymis cholesterol homeostasis and sperm fertilizing ability.”, Asian J. Androl., vol. 13 (1) , pp. 11–7, 2010.
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2009
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“Mammalian glutathione peroxidases control acquisition and maintenance of spermatozoa integrity.”, J. Anim. Sci., vol. 88 (4) , pp. 1321–31, 2009.
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“Epididymis seleno-independent glutathione peroxidase 5 maintains sperm DNA integrity in mice.”, J. Clin. Invest., vol. 119 (7) , pp. 2074–85, 2009.
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“[Role of reactive oxygen species (ROS) on human spermatozoa and male infertility].”, Gynecol Obstet Fertil, vol. 37 (6) , pp. 529–35, 2009.
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“LXR and ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell-specific manner.”, J. Lipid Res., vol. 50 (9) , pp. 1766–75, 2009.
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“LXR regulate cholesterol homeostasis in the proximal mouse epididymis.”, Folia Histochem. Cytobiol., vol. 47 (5) , pp. S75–9, 2009.
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2008
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“Protein composition of human epididymosomes collected during surgical vasectomy reversal: a proteomic and genomic approach.”, Hum. Reprod., vol. 23 (8) , pp. 1698–707, 2008.
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“GPX5, the selenium-independent glutathione peroxidase-encoding single copy gene is differentially expressed in mouse epididymis.”, Reprod. Fertil. Dev., vol. 20 (5) , pp. 615–25, 2008.
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2007
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“Identification of sperm antigens as a first step towards the generation of a contraceptive vaccine to decrease fossorial water vole Arvicola terrestris Scherman proliferations.”, Theriogenology, vol. 68 (5) , pp. 779–95, 2007.
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“Liver X receptors and epididymal epithelium physiology.”, Asian J. Androl., vol. 9 (4) , pp. 574–82, 2007.
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2004
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“Nuclear oxysterol receptors, LXRs, are involved in the maintenance of mouse caput epididymidis structure and functions.”, Journal of molecular endocrinology, vol. 33 (2) , pp. 361–75, 2004.
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