Team 05: Recombination and Maintenance of Genome Integrity

Our research concerns the cellular mechanisms assuring the maintenance of the genome, and its faithful transmission through development and reproduction. We work with the plant Arabidopsis, a biological model of particular interest for these studies due to the viability of the knockout mutants of many key genes, often lethal in animals. 

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Leader(s)
Publications

Research

On-going work falls under two headings:

1/ Recombination, DNA breaks and Chromosome stability. Living cells repair chomosome breaks through recombination and with new tools (in vivo DSB repair kinetics, endonucleases, fusion proteins,  cytometry, RNAseq...) we are studying the molecular mechanisms and control of the multiple pathways involved, including their roles at dysfunctional telomeres. Recent highlights include identification of a role for small RNAs in DNA break repair in Arabidopsis and in humans, signalling and repair of dysfunctional telomeres, a functional hierarchy of the four major recombination pathways and their roles in the transformation of plant cells by Agrobacterium T-DNA.

2/ Recombination and Chromosomal Pairing in Meiosis. We are studying mechanisms of recombination and homologous chromosome pairing in meiosis. Focussing on the roles of the Rad51 protein family, the MRN complex  and the structure-specific endonucleases, our recently published results show distinct requirements for these proteins in pairing of centromeres and of chromosome arms. Does this reflect heterochromatin/euchromatin differences, chromatin/DNA structure? Which molecular mechanisms are involved?

Research thematics

People

Last Name First Name Position Contact
Michel CHARBONNEAU profile picture CHARBONNEAU Michel Senior Research Fellow
Floriane CHERON profile picture CHERON Floriane Ph.D Student
Olivier DA INES profile picture DA INES Olivier Research Fellow
Maria Eugenia GALLEGO profile picture GALLEGO Maria Eugenia Professor
Nathalie GRANDIN profile picture GRANDIN Nathalie Research Fellow
Sebastien LAGEIX profile picture LAGEIX Sebastien Associate Professor
Etienne MOCHET profile picture MOCHET Etienne Intern
Valentine PETIOT profile picture PETIOT Valentine Ph.D Student
Heidi SERRA profile picture SERRA Heidi Research Fellow
Jeremy VERBEKE profile picture VERBEKE Jeremy Research Technician
Charles WHITE profile picture WHITE Charles Principal Investigator

Publications

  • 2022
    • A. Meschichi, L. Zhao, S. Reeck, C. White, O. Da Ines, A. Sicard, F. Pontvianne and S. Rosa, “The plant-specific DDR factor SOG1 increases chromatin mobility in response to DNA damage.”, EMBO Rep., pp. e54736, 2022.
    • O. Da Ines, J. Bazile, M. Gallego and C. White, “DMC1 attenuates RAD51-mediated recombination in Arabidopsis.”, PLoS Genet., vol. 18 (8) , pp. e1010322, 2022.
    • O. Da Ines, K. Choi, M. Pradillo and C. Lambing, “Editorial: Meiotic Recombination and DNA Repair: New Approaches to Solve Old Questions in Model and Non-model Plant Species.”, Front Plant Sci, vol. 13 , pp. 841402, 2022.
  • 2021
    • M. Martinez-Garcia, C. White, F. Franklin and E. Sanchez-Moran, “The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana.”, International journal of molecular sciences, vol. 22 (23) , 2021.
    • M. Hernandez Sanchez-Rebato, A. Bouatta, M. Gallego, C. White and O. Da Ines, “RAD54 is essential for RAD51-mediated repair of meiotic DSB in Arabidopsis.”, PLoS Genet., vol. 17 (5) , pp. e1008919, 2021.
    • P. Kuo, O. Da Ines and C. Lambing, “Rewiring Meiosis for Crop Improvement.”, Front Plant Sci, vol. 12 , pp. 708948, 2021.
  • 2020
    • P. Bourguet, L. Lopez-Gonzalez, A. Gomez-Zambrano, T. Pelissier, A. Hesketh, M. Potok, M. Pouch-Pelissier, M. Perez, O. Da Ines, D. Latrasse, C. White, S. Jacobsen, M. Benhamed and O. Mathieu, “DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis.”, Genome biology, vol. 21 (1) , pp. 283, 2020.
    • N. Grandin, M. Gallego, C. White and M. Charbonneau, “Inhibition of the alternative lengthening of telomeres pathway by subtelomeric sequences in Saccharomyces cerevisiae.”, DNA Repair (Amst.), vol. 96 , pp. 102996, 2020.
    • O. Da Ines, R. Michard, I. Fayos, G. Bastianelli, A. Nicolas, E. Guiderdoni, C. White and P. Sourdille, “Bread Wheat TaSPO11-1 exhibits evolutionary conserved function in meiotic recombination across distant plant species.”, Plant J., 2020.
    • F. Benyahya, I. Nadaud, O. Da Ines, H. Rimbert, C. White and P. Sourdille, “SPO11.2 is essential for programmed double strand break formation during meiosis in bread wheat (Triticum aestivum L.).”, Plant J., 2020.
  • 2019
    • N. Grandin, B. Pereira, C. Cohen, P. Billard, C. Dehais, C. Carpentier, A. Idbaih, F. Bielle, F. Ducray, D. Figarella-Branger, J. Delattre, M. Sanson, P. Lomonte, D. Poncet, P. Verrelle and M. Charbonneau, “The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas.”, Acta neuropathologica communications, vol. 7 (1) , pp. 175, 2019.
    • T. Hirakawa, K. Kuwata, M. Gallego, C. White, M. Nomoto, Y. Tada and S. Matsunaga, “LSD1-LIKE1-mediated H3K4me2 demethylation is required for homologous recombination repair.”, Plant physiology, 2019.
    • O. Calvo, N. Grandin, A. Jordan-Pla, E. Minambres, N. Gonzalez-Polo, J. Perez-Ortin and M. Charbonneau, “The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription.”, Nucleic Acids Res., vol. 47 (12) , pp. 6250–6268, 2019.
  • 2018
  • 2017
    • E. Balc'h, N. Grandin, M. Demattei, S. Guyetant, A. Tallet, J. Pages, M. Ouaissi, T. Lecomte and M. Charbonneau, “Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis.”, International journal of molecular sciences, vol. 18 (9) , 2017.
    • A. Fogli, M. Demattei, L. Corset, C. Vaurs-Barriere, E. Chautard, J. Biau, J. Kemeny, C. Godfraind, B. Pereira, T. Khalil, N. Grandin, P. Arnaud, M. Charbonneau and P. Verrelle, “Detection of the alternative lengthening of telomeres pathway in malignant gliomas for improved molecular diagnosis.”, Journal of neuro-oncology, 2017.
    • T. Hirakawa, J. Hasegawa, C. White and S. Matsunaga, “RAD54 forms DNA repair foci in response to DNA damage in living plant cells.”, Plant J., 2017.
    • G. Singh, O. Da Ines, M. Gallego and C. White, “Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis.”, PLoS ONE, vol. 12 (8) , pp. e0183006, 2017.
  • 2016
    • C. Collonnier, A. Epert, K. Mara, F. Maclot, A. Guyon-Debast, F. Charlot, C. White, D. Schaefer and F. Nogue, “CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the moss Physcomitrella patens.”, Plant Biotechnol. J., 2016.
    • J. Pedroza-Garcia, S. Domenichini, C. Mazubert, M. Bourge, C. White, E. Hudik, R. Bounon, Z. Tariq, E. Delannoy, I. Del Olmo, M. Pineiro, J. Jarillo Quiroga, C. Bergounioux, M. Benhamed and C. Raynaud, “Role of the Polymerase sub-unit DPB2 in DNA replication, cell cycle regulation and DNA damage response in Arabidopsis.”, Nucleic Acids Res., 2016.
    • K. Richter, H. Serra, C. White and H. Jeske, “The recombination mediator RAD51D promotes geminiviral infection.”, Virology, vol. 493 , pp. 113–127, 2016.
    • M. Olivier, O. Da Ines, S. Amiard, H. Serra, C. Goubely, C. White and M. Gallego, “The Structure-Specific Endonucleases MUS81 and SEND1 Are Essential for Telomere Stability in Arabidopsis.”, Plant Cell, vol. 28 (1) , pp. 74–86, 2016.
  • 2015
    • O. Da Ines and C. White, “Centromere Associations in Meiotic Chromosome Pairing.”, Annual review of genetics, 2015.
    • R. Eid, M. Demattei, H. Episkopou, C. Auge-Gouillou, A. Decottignies, N. Grandin and M. Charbonneau, “Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells.”, Molecular and cellular biology, vol. 35 (16) , pp. 2818–30, 2015.
    • V. Muchova, S. Amiard, I. Mozgova, M. Dvorackova, M. Gallego, C. White and J. Fajkus, “Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants.”, Plant J., vol. 81 (2) , pp. 198–209, 2015.
    • P. George, S. Jensen, R. Pogorelcnik, J. Lee, Y. Xing, E. Brasset, C. Vaury and I. Sharakhov, “Increased production of piRNAs from euchromatic clusters and genes in Anopheles gambiae compared with Drosophila melanogaster.”, Epigenetics & chromatin, vol. 8 , pp. 50, 2015.
    • P. Merle, M. Gueugneau, M. Teulade-Fichou, M. Muller-Barthelemy, S. Amiard, E. Chautard, C. Guetta, V. Dedieu, Y. Communal, J. Mergny, M. Gallego, C. White, P. Verrelle and A. Tchirkov, “Highly efficient radiosensitization of human glioblastoma and lung cancer cells by a G-quadruplex DNA binding compound.”, Scientific reports, vol. 5 , pp. 16255, 2015.
  • 2014
    • S. Amiard, M. Olivier, E. Allain, K. Choi, R. Smith-Unna, I. Henderson, C. White and M. Gallego, “Telomere stability and development of ctc1 mutants are rescued by inhibition of EJ recombination pathways in a telomerase-dependent manner.”, Nucleic Acids Res., vol. 42 (19) , pp. 11979–91, 2014.
    • P. Kalhorzadeh, Z. Hu, T. Cools, S. Amiard, E. Willing, N. De Winne, K. Gevaert, G. De Jaeger, K. Schneeberger, C. White and L. De Veylder, “Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability.”, Plant Cell, vol. 26 (9) , pp. 3680–92, 2014.
    • O. Da Ines, M. Gallego and C. White, “Recombination-independent mechanisms and pairing of homologous chromosomes during meiosis in plants.”, Mol Plant, vol. 7 (3) , pp. 492–501, 2014.
    • I. Mestiri, F. Norre, M. Gallego and C. White, “Multiple host-cell recombination pathways act in Agrobacterium-mediated transformation of plant cells.”, Plant J., vol. 77 (4) , pp. 511–20, 2014.
    • S. Amiard, O. Da Ines, M. Gallego and C. White, “Responses to telomere erosion in plants.”, PLoS ONE, vol. 9 (1) , pp. e86220, 2014.
  • 2013
    • H. Serra, O. Da Ines, F. Degroote, M. Gallego and C. White, “Roles of XRCC2, RAD51B and RAD51D in RAD51-independent SSA recombination.”, PLoS Genet., vol. 9 (11) , pp. e1003971, 2013.
    • O. Da Ines, F. Degroote, S. Amiard, C. Goubely, M. Gallego and C. White, “Effects of XRCC2 and RAD51B mutations on somatic and meiotic recombination in Arabidopsis thaliana.”, Plant J., vol. 74 (6) , pp. 959–70, 2013.
    • K. Prado, Y. Boursiac, C. Tournaire-Roux, J. Monneuse, O. Postaire, O. Da Ines, A. Schaffner, S. Hem, V. Santoni and C. Maurel, “Regulation of Arabidopsis leaf hydraulics involves light-dependent phosphorylation of aquaporins in veins.”, Plant Cell, vol. 25 (3) , pp. 1029–39, 2013.
    • O. Da Ines and C. White, “Gene Site-Specific Insertion in Plants”, Site-directed insertion of transgenes, pp. 287–315, 2013.
    • O. Da Ines, F. Degroote, C. Goubely, S. Amiard, M. Gallego and C. White, “Meiotic recombination in Arabidopsis is catalysed by DMC1, with RAD51 playing a supporting role.”, PLoS Genet., vol. 9 (9) , pp. e1003787, 2013.
    • S. Amiard, M. Gallego and C. White, “Signaling of double strand breaks and deprotected telomeres in Arabidopsis.”, Front Plant Sci, vol. 4 , pp. 405, 2013.
  • 2012
    • A. Ayar, S. Wehrkamp-Richter, J. Laffaire, S. Le Goff, J. Levy, S. Chaignon, H. Salmi, A. Lepicard, C. Sallaud, M. Gallego, C. White and W. Paul, “Gene targeting in maize by somatic ectopic recombination.”, Plant Biotechnol. J., vol. 11 (3) , pp. 305–14, 2012.
    • B. Peret, G. Li, J. Zhao, L. Band, U. Voss, O. Postaire, D. Luu, O. Da Ines, I. Casimiro, M. Lucas, D. Wells, L. Lazzerini, P. Nacry, J. King, O. Jensen, A. Schaffner, C. Maurel and M. Bennett, “Auxin regulates aquaporin function to facilitate lateral root emergence.”, Nature cell biology, vol. 14 (10) , pp. 991–8, 2012.
    • W. Wei, Z. Ba, M. Gao, Y. Wu, Y. Ma, S. Amiard, C. White, J. Rendtlew Danielsen, Y. Yang and Y. Qi, “A role for small RNAs in DNA double-strand break repair.”, Cell, vol. 149 (1) , pp. 101–12, 2012.
    • O. Da Ines, K. Abe, C. Goubely, M. Gallego and C. White, “Differing requirements for RAD51 and DMC1 in meiotic pairing of centromeres and chromosome arms in Arabidopsis thaliana.”, PLoS Genet., vol. 8 (4) , pp. e1002636, 2012.
  • 2011
    • S. Amiard, A. Depeiges, E. Allain, C. White and M. Gallego, “Arabidopsis ATM and ATR kinases prevent propagation of genome damage caused by telomere dysfunction.”, Plant Cell, vol. 23 (12) , pp. 4254–65, 2011.
    • C. Charbonnel, E. Allain, M. Gallego and C. White, “Kinetic analysis of DNA double-strand break repair pathways in Arabidopsis.”, DNA Repair (Amst.), vol. 10 (6) , pp. 611–9, 2011.
    • S. Amiard, C. White and M. Gallego, “Recombination proteins and telomere stability in plants.”, Curr. Protein Pept. Sci., vol. 12 (2) , pp. 84–92, 2011.
    • Z. Kevei, M. Baloban, O. Da Ines, H. Tiricz, A. Kroll, K. Regulski, P. Mergaert and E. Kondorosi, “Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.”, PLoS ONE, vol. 6 (6) , pp. e20618, 2011.
  • 2010
    • S. Amiard, C. Charbonnel, E. Allain, A. Depeiges, C. White and M. Gallego, “Distinct roles of the ATR kinase and the Mre11-Rad50-Nbs1 complex in the maintenance of chromosomal stability in Arabidopsis.”, Plant Cell, vol. 22 (9) , pp. 3020–33, 2010.
    • C. Charbonnel, M. Gallego and C. White, “Xrcc1-dependent and Ku-dependent DNA double-strand break repair kinetics in Arabidopsis plants.”, Plant J., vol. 64 (2) , pp. 280–90, 2010.
    • O. Da Ines, W. Graf, K. Franck, A. Albert, J. Winkler, H. Scherb, W. Stichler and A. Schaffner, “Kinetic analyses of plant water relocation using deuterium as tracer - reduced water flux of Arabidopsis pip2 aquaporin knockout mutants.”, Plant biology (Stuttgart, Germany), vol. 12 Suppl 1 , pp. 129–39, 2010.
  • 2009
    • M. Vanstraelen, M. Baloban, O. Da Ines, A. Cultrone, T. Lammens, V. Boudolf, S. Brown, L. De Veylder, P. Mergaert and E. Kondorosi, “APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root.”, Proc. Natl. Acad. Sci. U.S.A., vol. 106 (28) , pp. 11806–11, 2009.
    • J. Vannier, A. Depeiges, C. White and M. Gallego, “ERCC1/XPF protects short telomeres from homologous recombination in Arabidopsis thaliana.”, PLoS Genet., vol. 5 (2) , pp. e1000380, 2009.
  • 2008
    • J. Kozak, C. West, C. White, J. da Costa-Nunes and K. Angelis, “Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance.”, DNA Repair (Amst.), vol. 8 (3) , pp. 413–9, 2008.
    • C. White, “News from Arabidopsis on the meiotic roles of Blap75/Rmi1 and Top3alpha.”, PLoS Genet., vol. 4 (12) , pp. e1000306, 2008.
    • S. Wehrkamp-Richter, F. Degroote, J. Laffaire, W. Paul, P. Perez and G. Picard, “Characterisation of a new reporter system allowing high throughput in planta screening for recombination events before and after controlled DNA double strand break induction.”, Plant physiol. biochem., vol. 47 (4) , pp. 248–55, 2008.
    • A. Sommer, B. Geist, O. Da Ines, R. Gehwolf, A. Schaffner and G. Obermeyer, “Ectopic expression of Arabidopsis thaliana plasma membrane intrinsic protein 2 aquaporins in lily pollen increases the plasma membrane water permeability of grain but not of tube protoplasts.”, The New phytologist, vol. 180 (4) , pp. 787–97, 2008.