Team 08: Diversification of muscle and heart cells in normal and pathological conditions

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Our research group is using Drosophila model to study molecular mechanisms that control diversification of muscle and cardiac cells during normal development and in pathological contexts. Our main goal is to apply the power of the Drosophila model and to develop cell-specific genomic approaches for analyzing genetic control of diversification processes. We also develop applied projects dedicated to make a link between pathogenesis of muscular diseases affecting muscle subsets and mechanisms governing diversification of muscle types.

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Research

During last years using genome-wide strategies we identified the first target genes of cell identity factors Ladybird and determined the first molecular code of acting downstream realisator genes. Collaboration with U. Straehle, KIT Karlsruhe has also led to the identification of developmental muscle functions of metabolic genes and in particular of those encoding glycolytic enzymes. In parallel we also defined signaling events that are required for specification and reactivation of Drosophila muscle stem cells and a gene cascade governing morphogenesiss of the cardiac outflow and diversification of Eve-positive pericardial cells.

Another axis of our research is dedicated to build Drosophila models for human muscular diseases. Within this frame we have generated a new Drosophila model of Myotonic Dystrophy type 1 (DM1) and performed phenotypic and transcriptomic analyses. We also identified involvement of the dCryAB gene from the sHsp family in the Drosophila model of Desminopathy and more recently characterised the role of Drosophila CELF1 orthologue in DM1.

A part of our team works actually on technological projects related to transgenesis and CRISPR/Cas9 genome editing. This platform activity with potential médicale applications is: funded by the Infrastructure TEFOR and strategic AFM-Téléthon grants.

Altogether research projects of the team are dedicated to:

  • Fundamental research : “Studying diversification of cardiac and muscle cells using genome wide cell specific approaches »
  • Applied research : « Generation and characterisation of Drosophila models for human muscular diseases »
  • Platform activities (www.fly-facility.com): "Transgenesis and CRISPRs genome editing for médicale applications"

Research thematics

People

Last Name First Name Position Contact
Sandy AL-HAYEK profile picture AL-HAYEK Sandy Post-doctoral Fellow
Fabiana CERQUEIRA CAMPOS profile picture CERQUEIRA CAMPOS Fabiana Post-doctoral Fellow
Krzysztof JAGLA profile picture JAGLA Krzysztof Principal Investigator
Teresa JAGLA profile picture JAGLA Teresa Research Engineer
Guillaume JUNION profile picture JUNION Guillaume Research Fellow
Margot LUGOBONI profile picture LUGOBONI Margot Ph.D Student
Blandine MOUCAUD profile picture MOUCAUD Blandine Ph.D Student
Lea MOUTY profile picture MOUTY Lea Research Technician
Elodie PRINCE profile picture PRINCE Elodie Post-doctoral Fellow
Cedric SOLER profile picture SOLER Cedric Associate Professor
Anissa SOUIDI profile picture SOUIDI Anissa Ph.D Student
Monika ZMOJDZIAN profile picture ZMOJDZIAN Monika Post-doctoral Fellow

Publications

  • 2021
    • A. Souidi and K. Jagla, “Drosophila Heart as a Model for Cardiac Development and Diseases.”, Cells, vol. 10 (11) , 2021.
    • C. Dondi, B. Bertin, J. Da Ponte, I. Wojtowicz, K. Jagla and G. Junion, “A polarized nucleus-cytoskeleton-ECM connection in migrating cardioblasts controls heart tube formation in Drosophila.”, Development, vol. 148 (15) , 2021.
    • J. Akhtar, Y. Renaud, S. Albrecht, Y. Ghavi-Helm, J. Roignant, M. Silies and G. Junion, “m(6)A RNA methylation regulates promoter- proximal pausing of RNA polymerase II.”, Molecular cell, 2021.
    • B. Bertin, Y. Renaud, T. Jagla, G. Lavergne, C. Dondi, J. Da Ponte, G. Junion and K. Jagla, “Gelsolin and dCryAB act downstream of muscle identity genes and contribute to preventing muscle splitting and branching in Drosophila.”, Scientific reports, vol. 11 (1) , pp. 13197, 2021.
    • M. Zmojdzian and K. Jagla, “The relationship between muscle stem cells and motor neurons.”, Cell. Mol. Life Sci., vol. 78 (12) , pp. 5043–5049, 2021.
    • M. Dubinska-Magiera, M. Migocka-Patrzalek, D. Lewandowski, M. Daczewska and K. Jagla, “Zebrafish as a Model for the Study of Lipid-Lowering Drug-Induced Myopathies.”, International journal of molecular sciences, vol. 22 (11) , 2021.
    • Q. Laurichesse, B. Moucaud, L. Laddada, Y. Renaud, K. Jagla and C. Soler, “Transcriptomic and Genetic Analyses Identify the Kruppel-Like Factor Dar1 as a New Regulator of Tube-Shaped Long Tendon Development.”, Frontiers in cell and developmental biology, vol. 9 , pp. 747563, 2021.
  • 2020
    • P. Poovathumkadavil and K. Jagla, “Genetic Control of Muscle Diversification and Homeostasis: Insights from Drosophila.”, Cells, vol. 9 (6) , 2020.
    • G. Lavergne, M. Zmojdzian, J. Da Ponte, G. Junion and K. Jagla, “Drosophila adult muscle precursor cells contribute to motor axon pathfinding and proper innervation of embryonic muscles.”, Development, vol. 147 (4) , 2020.
    • R. Aradhya and K. Jagla, “Insulin-dependent Non-canonical Activation of Notch in Drosophila: A Story of Notch-Induced Muscle Stem Cell Proliferation.”, Advances in experimental medicine and biology, vol. 1227 , pp. 131–144, 2020.
  • 2019
  • 2018
    • A. Souidi, M. Zmojdzian and K. Jagla, “Dissecting Pathogenetic Mechanisms and Therapeutic Strategies in Drosophila Models of Myotonic Dystrophy Type 1.”, International journal of molecular sciences, vol. 19 (12) , 2018.
    • T. Jagla, M. Dubinska-Magiera, P. Poovathumkadavil, M. Daczewska and K. Jagla, “Developmental Expression and Functions of the Small Heat Shock Proteins in Drosophila.”, International journal of molecular sciences, vol. 19 (11) , 2018.
    • J. Jablonska, M. Dubinska-Magiera, T. Jagla, K. Jagla and M. Daczewska, “Drosophila Hsp67Bc hot-spot variants alter muscle structure and function.”, Cell. Mol. Life Sci., 2018.
    • L. Picchio, V. Legagneux, S. Deschamps, Y. Renaud, S. Chauveau, L. Paillard and K. Jagla, “Bruno-3 regulates sarcomere component expression and contributes to muscle phenotypes of myotonic dystrophy type 1.”, Disease models & mechanisms, vol. 11 (5) , 2018.
  • 2017
    • M. Zmojdzian, S. de Joussineau, J. Da Ponte and K. Jagla, “Distinct subsets of Eve pericardial cells stabilise cardiac outflow and contribute to Hox-triggered heart morphogenesis in Drosophila.”, Development, 2017.
    • K. Jagla, B. Kalman, T. Boudou, S. Henon and S. Batonnet-Pichon, “Beyond mice: Emerging and transdisciplinary models for the study of early-onset myopathies.”, Seminars in cell & developmental biology, vol. 64 , pp. 171–180, 2017.
    • G. Lavergne, C. Soler, M. Zmojdzian and K. Jagla, “Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors.”, Meth. Mol. Biol., vol. 1556 , pp. 103–116, 2017.
  • 2016
    • D. Seyres, Y. Ghavi-Helm, G. Junion, O. Taghli-Lamallem, C. Guichard, L. Roder, C. Girardot, E. Furlong and L. Perrin, “Identification and in silico modeling of enhancers reveals new features of the cardiac differentiation network.”, Development, vol. 143 (23) , pp. 4533–4542, 2016.
    • M. Dubinska-Magiera, M. Daczewska, A. Lewicka, M. Migocka-Patrzalek, J. Niedbalska-Tarnowska and K. Jagla, “Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function.”, International journal of molecular sciences, vol. 17 (11) , 2016.
    • A. Mbodj, E. Gustafson, L. Ciglar, G. Junion, A. Gonzalez, C. Girardot, L. Perrin, E. Furlong and D. Thieffry, “Qualitative Dynamical Modelling Can Formally Explain Mesoderm Specification and Predict Novel Developmental Phenotypes.”, PLoS computational biology, vol. 12 (9) , pp. e1005073, 2016.
    • O. Taghli-Lamallem, E. Plantie and K. Jagla, “Drosophila in the Heart of Understanding Cardiac Diseases: Modeling Channelopathies and Cardiomyopathies in the Fruitfly.”, Journal of cardiovascular development and disease, vol. 3 (1) , 2016.
    • C. Soler, L. Laddada and K. Jagla, “Coordinated Development of Muscles and Tendon-Like Structures: Early Interactions in the Drosophila Leg.”, Frontiers in physiology, vol. 7 , pp. 22, 2016.
  • 2015
  • 2014
    • M. Dubinska-Magiera, J. Jablonska, J. Saczko, J. Kulbacka, T. Jagla and M. Daczewska, “Contribution of small heat shock proteins to muscle development and function.”, FEBS Lett., vol. 588 (4) , pp. 517–30, 2014.
    • O. Taghli-Lamallem, K. Jagla, J. Chamberlain and R. Bodmer, “Mechanical and non-mechanical functions of Dystrophin can prevent cardiac abnormalities in Drosophila.”, Experimental gerontology, vol. 49 , pp. 26–34, 2014.
  • 2013
    • V. Tixier, L. Bataille, C. Etard, T. Jagla, M. Weger, J. Daponte, U. Strahle, T. Dickmeis and K. Jagla, “Glycolysis supports embryonic muscle growth by promoting myoblast fusion.”, Proc. Natl. Acad. Sci. U.S.A., vol. 110 (47) , pp. 18982–7, 2013.
    • A. Mbodj, G. Junion, C. Brun, E. Furlong and D. Thieffry, “Logical modelling of Drosophila signalling pathways.”, Mol Biosyst, vol. 9 (9) , pp. 2248–58, 2013.
    • M. Zmojdzian and K. Jagla, “Tailup plays multiple roles during cardiac outflow assembly in Drosophila.”, Cell Tissue Res., vol. 354 (2) , pp. 639–45, 2013.
    • L. Picchio, E. Plantie, Y. Renaud, P. Poovthumkadavil and K. Jagla, “Novel Drosophila model of myotonic dystrophy type 1: phenotypic characterization and genome-wide view of altered gene expression.”, Hum. Mol. Genet., vol. 22 (14) , pp. 2795–810, 2013.
  • 2012
    • G. Junion, M. Spivakov, C. Girardot, M. Braun, E. Gustafson, E. Birney and E. Furlong, “A transcription factor collective defines cardiac cell fate and reflects lineage history.”, Cell, vol. 148 (3) , pp. 473–86, 2012.
    • G. Junion and K. Jagla, “ChIP-enriched in silico targets (ChEST), a ChIP-on-chip approach applied to analyzing skeletal muscle genes.”, Meth. Mol. Biol., vol. 798 , pp. 543–53, 2012.
    • C. de Joussineau, L. Bataille, T. Jagla and K. Jagla, “Diversification of muscle types in Drosophila: upstream and downstream of identity genes.”, Curr. Top. Dev. Biol., vol. 98 , pp. 277–301, 2012.
  • 2011
  • 2010
    • L. Bataille, I. Delon, J. Da Ponte, N. Brown and K. Jagla, “Downstream of identity genes: muscle-type-specific regulation of the fusion process.”, Dev. Cell, vol. 19 (2) , pp. 317–28, 2010.
    • V. Tixier, L. Bataille and K. Jagla, “Diversification of muscle types: recent insights from Drosophila.”, Exp. Cell Res., vol. 316 (18) , pp. 3019–27, 2010.
    • N. Figeac, T. Jagla, R. Aradhya, J. Da Ponte and K. Jagla, “Drosophila adult muscle precursors form a network of interconnected cells and are specified by the rhomboid-triggered EGF pathway.”, Development, vol. 137 (12) , pp. 1965–73, 2010.
    • M. Daczewska, L. Picchio, T. Jagla, N. Figeac and K. Jagla, “Muscle development and regeneration in normal and pathological conditions: learning from Drosophila.”, Curr. Pharm. Des., vol. 16 (8) , pp. 929–41, 2010.
    • D. Muller, T. Jagla, L. Bodart, N. Jahrling, H. Dodt, K. Jagla and M. Frasch, “Regulation and functions of the lms homeobox gene during development of embryonic lateral transverse muscles and direct flight muscles in Drosophila.”, PLoS ONE, vol. 5 (12) , pp. e14323, 2010.
  • 2009
    • H. Meyer, M. Panz, M. Zmojdzian, K. Jagla and A. Paululat, “Neprilysin 4, a novel endopeptidase from Drosophila melanogaster, displays distinct substrate specificities and exceptional solubility states.”, J. Exp. Biol., vol. 212 (Pt 22) , pp. 3673–83, 2009.
    • A. Kacperczyk, T. Jagla and M. Daczewska, “Pax-3 and Pax-7 label muscle progenitor cells during myotomal myogenesis in Coregonus lavaretus (Teleostei: Coregonidae).”, Anat Histol Embryol, vol. 38 (6) , pp. 411–8, 2009.
  • 2008
    • C. Medioni, M. Astier, M. Zmojdzian, K. Jagla and M. Semeriva, “Genetic control of cell morphogenesis during Drosophila melanogaster cardiac tube formation.”, J. Cell Biol., vol. 182 (2) , pp. 249–61, 2008.
    • M. Zmojdzian, J. Da Ponte and K. Jagla, “Cellular components and signals required for the cardiac outflow tract assembly in Drosophila.”, Proc. Natl. Acad. Sci. U.S.A., vol. 105 (7) , pp. 2475–80, 2008.