Our primary interest is to understand the role of Environmental Cues in Axis Morphogenesis.
To this end, we use cutting-edge technologies to study axis dynamics from the tissue to the transcriptional levels through a newly discovered cell type, the NMPs.
We use the chicken as a model for studying axis morphogenesis. While birds look quite different from humans, early developmental stages are very similar (right picture)
Our ability to manipulate and challenge the embryo directly in the egg during axis development allows us to pose relevant questions to understand human congenital malformations linked to axial development. We employ cutting-edge techniques of live imaging, lineage tracing, quantitative analysis, and single-cell RNA sequencing to uncover how NMP for the body of vertebrates.
We identified the NeuroMesodermal Progenitors (NMP) in the chick model as a population of cells in the Anterior Primitive Streak (PS) that become the main progenitors in the tailed (Tb) to form the body axis (Left)
Once in the tailed, the bipotent NMP gives rise to cells in Bothe the Neural Tube (NT) & preSomitic Mesoderm (PSM) that are the embryonic precursors of the Spinal Cord & Musculoskeletal tissue, respectively. (Right)
Current Projects : Role of Environmental cues in …
… the fate and lineage of NMPs
Using individual NMP color labeling, we can study the fate and lineage of the NMPs in vivo.
NT: Neural Tube, S: Somite, HL: HindLimb.
… the dynamics of NMPs
NMPs morph into the tailbud to become the main progenitors of the body axis through peculiar cell dynamics i.e. low ingression, low convergence and high cell division.
… the cellular states forming the body axis
Single-cell analysis of the cells forming the posterior body identifies the NMP and their contribution to both the Neural and Mesodermal lineages in chick and mouse models.
YES!!! We Speak English in the lab (and a little bit of French)
We seek to provide an inclusive lab environment with lambastes from diverse backgrounds and experiences.
Past Lab Members
Pauline DUMONT – Graduate student – Master BIP/UCA (2022)
Adrien CHARTROULE – Undergraduate student – DUT/UCA (2022)
Sophie PHAN – Undergraduate student – L3 Life Sciences Bachelor/UCA (2022)
Emma DE CARVALHO – Undergraduate student – BTS/ (2022)
Publié le 28 Oct 2021 dans Current Opinion in Cell Biology , vol. 73 - pp 133-140
Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo
Publié le 06 Juil 2021 dans eLife Sciences
Charlene Guillot , Yannis Djeffal, Arthur Michaut, Brian Rabe, Olivier Pourquié
Publié le 14 Août 2019 dans Annu Rev Cell Dev Biol - pp 259–283
Alessandro Mongera , Michaut Arthur, Guillot Charlene, Xiong Fengzhu, and Pourquié Olivier
PAPC couples the segmentation clock to somite morphogenesis by regulating N-cadherin dependent adhesion
Publié le 19 Déc 2016 dans Development - pp 664-676
JéromeChal & CharleǹeGuillot , OlivierPourquié