Research Team

reclab – recombination and the maintenance of genome integrity

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HomeTeamsreclab – recombination and the maintenance of genome integrity
Working with the plant, Arabidopsis, and the yeast, S. cerevisiae, we study the molecular and cellular mechanisms assuring the maintenance of the genome, and its faithful transmission through development and reproduction.

Funders

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2022-06 reclab

Research

 

 

1/ Recombination, DNA breaks and Chromosome stability  (Contacts: Sébastien Lageix, Maria Gallego, Charles White)

Chromosome breaks occur in all living cells and, by separating all or part of a chromosome arm from its centromere, can result in the loss of hundreds or thousands of genes.  Multiple, highly conserved recombination pathways efficiently repair chomosome breaks, and the specific repair mechanism used determines the outcome of the repair.  With new tools (in vivo DSB repair kinetics, CRISPR endonucleases, microscopy, cytometry, NGS sequencing, Nanopore …) we are studying the impact of genetic and epigentic contexts of chromosome breaks on the molecular mechanisms involved in their repair in mitosis and meiosis. 

 

 

2/  RAD51/DMC1 and the control of Meiotic Recombination. (Contact: Olivier Da Ines).

 Key to successful meiosis is DNA double strand break (DSB) formation followed by homologous recombination (HR). The latter depends on successful search and invasion of homologous DNA repair templates. For this crucial process organisms across phyla rely on the cooperation of two related recombinase proteins: RAD51 and DMC1. RAD51 is the sole recombinase protein acting during mitotic recombination. DMC1 is the leading strand exchange protein in meiosis with RAD51 playing a crucial supportive role yet having its own strand-exchange activity downregulated. Importantly, recombinases depend on accessory factors that determine their activity (many of which remain unidentified in plants to date) and the entire process is embedded in meiosis-specific chromatin architecture. Understanding why eukaryotes have two recombinases and how are their activities regulated remains elusive and is the main objective of this project.

 

 

3/ Meiotic adaptation to allopolyploidy. (Contact: Heïdi Serra) 

Allopolyploidy resulting from hybridization between related species is ubiquitous in the evolutionary history of plants. All newly formed allopolyploid organisms face the challenge of chromosome segregation during meiosis due to the two (or more) sets of non-identical related chromosomes (called homoeologues) inherited from each progenitor. Using the allotetraploid Arabidopsis suecica as a study model and a set of cytogenetic, genomic and epigenomic approaches, we are studying the processes allowing meiotic adaptation to allopolyploidy, and in particular the molecular mechanisms that control the pairing and recombination between homoeologous chromosomes.

 

 

4/  Telomeric and non-telomeric roles of the CST complex.  (Contacts: Michel Charbonneau, Nathalie Grandin) 

In all tumor cells, uncontrolled proliferation is associated with their capacity to maintain functional telomeres, the extremitites of linear chromosomes. Telomeric DNA is replicated by a specialized reverse transcriptase, telomerase. In addition, telomeres recruit specialized proteins that form a protective cap which prevents telomeres from being degraded by DNA modification and repair enzymes. We currently study in a classical model system, the yeast Saccharomyces cerevisiae, the Cdc13, Stn1 and Ten1 telomeric proteins, which form the evolutionary conserved CST complex, and are essential for ensuring telomere end protection and telomerase-mediated telomere length regulation, as well as in non-telomeric functions such as regulation of transcription and mitotic spindle stability.

 

Members

Publications

70 publications
2024
2022

The plant-specific DDR factor SOG1 increases chromatin mobility in response to DNA damage.

Published on 06 Dec 2022 in EMBO reports , vol. 23 - pp e54736

Meschichi A, Zhao L, Reeck S, White C , Da Ines O , Sicard A, Pontvianne F, Rosa S

DMC1 attenuates RAD51-mediated recombination in Arabidopsis.

Published on 30 Aug 2022 in PLoS genetics , vol. 18 - pp e1010322

Da Ines O , Bazile J , Gallego ME , White CI

Generation of Deletion Lines in Allohexaploid Bread Wheat.

Published on 24 Apr 2022 in Methods in molecular biology (Clifton, N.J.) , vol. 2484 - pp 183-199

Serra H , Svačina R, Bartoš J, Sourdille P

Editorial: Meiotic Recombination and DNA Repair: New Approaches to Solve Old Questions in Model and Non-model Plant Species.

Published on 09 Feb 2022 in Frontiers in plant science , vol. 13 - pp 841402

Da Ines O , Choi K, Pradillo M, Lambing C

2021

The TeloDIAG: how telomeric parameters can help in glioma rapid diagnosis and liquid biopsy approaches.

Published on 30 Dec 2021 in Annals of oncology : official journal of the European Society for Medical Oncology , vol. 32 - pp 1608-1617

Billard P, Guerriau C, Carpentier C, Juillard F, Grandin N , Lomonte P, Kantapareddy P, Dufay N, Barritault M, Rimokh R, Verrelle P, Maucort-Boulch D, Figarella-Branger D, Ducray F, Dehais C, Charbonneau M , Meyronet D, Poncet DA, POLA network

The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana.

Published on 04 Dec 2021 in International journal of molecular sciences , vol. 22

Martinez-Garcia M, White CI , Franklin FCH, Sanchez-Moran E

Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis.

Published on 17 Aug 2021 in Proceedings of the National Academy of Sciences of the United States of America , vol. 118

Zhu L, Fernández-Jiménez N, Szymanska-Lejman M, Pelé A, Underwood CJ, Serra H , Lambing C, Dluzewska J, Bieluszewski T, Pradillo M, Henderson IR, Ziolkowski PA

Rewiring Meiosis for Crop Improvement.

Published on 05 Aug 2021 in Frontiers in plant science , vol. 12 - pp 708948

Kuo P, Da Ines O , Lambing C

Ph2 encodes the mismatch repair protein MSH7-3D that inhibits wheat homoeologous recombination.

Published on 05 Feb 2021 in Nature communications , vol. 12 - pp 803

Serra H , Svačina R, Baumann U, Whitford R, Sutton T, Bartoš J, Sourdille P

2020

DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis.

Published on 25 Nov 2020 in Genome biology , vol. 21 - pp 283

Bourguet P , López-González L, Gómez-Zambrano Á, Pélissier T , Hesketh A , Potok ME, Pouch-Pélissier MN , Perez M, Da Ines O , Latrasse D, White CI , Jacobsen SE, Benhamed M, Mathieu O

SPO11.2 is essential for programmed double-strand break formation during meiosis in bread wheat (Triticum aestivum L.).

Published on 30 Sep 2020 in The Plant journal : for cell and molecular biology , vol. 104 - pp 30-43

Benyahya F, Nadaud I, Da Ines O , Rimbert H, White C , Sourdille P

Bread wheat TaSPO11-1 exhibits evolutionarily conserved function in meiotic recombination across distant plant species.

Published on 30 Sep 2020 in The Plant journal : for cell and molecular biology , vol. 103 - pp 2052-2068

Da Ines O , Michard R, Fayos I, Bastianelli G, Nicolas A, Guiderdoni E, White C , Sourdille P

Development of Deletion Lines for Chromosome 3D of Bread Wheat.

Published on 28 Jan 2020 in Frontiers in plant science , vol. 10 - pp 1756

Svačina R, Karafiátová M, Malurová M, Serra H , Vítek D, Endo TR, Sourdille P, Bartoš J

2019

The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas.

Published on 09 Nov 2019 in Acta neuropathologica communications , vol. 7 - pp 175

Grandin N , Pereira B, Cohen C, Billard P, Dehais C, Carpentier C, Idbaih A, Bielle F, Ducray F, Figarella-Branger D, Delattre JY, Sanson M, Lomonte P, Poncet D, Verrelle P, Charbonneau M , POLA network

LSD1-LIKE1-Mediated H3K4me2 Demethylation Is Required for Homologous Recombination Repair.

Published on 30 Oct 2019 in Plant physiology , vol. 181 - pp 499-509

Hirakawa T, Kuwata K, Gallego ME , White CI , Nomoto M, Tada Y, Matsunaga S

The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription.

Published on 09 Jul 2019 in Nucleic acids research , vol. 47 - pp 6250-6268

Calvo O, Grandin N , Jordán-Pla A, Miñambres E, González-Polo N, Pérez-Ortín JE, Charbonneau M

2018

Interhomolog polymorphism shapes meiotic crossover within the Arabidopsis RAC1 and RPP13 disease resistance genes.

Published on 30 Dec 2018 in PLoS genetics , vol. 14 - pp e1007843

Serra H , Choi K, Zhao X, Blackwell AR, Kim J, Henderson IR

The Linker Histone GH1-HMGA1 Is Involved in Telomere Stability and DNA Damage Repair.

Published on 30 May 2018 in Plant physiology , vol. 177 - pp 311-327

Charbonnel C , Rymarenko O, Da Ines O , Benyahya F, White CI , Butter F, Amiard S

Epigenetic activation of meiotic recombination near Arabidopsis thaliana centromeres via loss of H3K9me2 and non-CG DNA methylation.

Published on 30 Apr 2018 in Genome research , vol. 28 - pp 519-531

Underwood CJ, Choi K, Lambing C, Zhao X, Serra H , Borges F, Simorowski J, Ernst E, Jacob Y, Henderson IR, Martienssen RA

Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.

Published on 30 Apr 2018 in Genome research , vol. 28 - pp 532-546

Choi K, Zhao X, Tock AJ, Lambing C, Underwood CJ, Hardcastle TJ, Serra H , Kim J, Cho HS, Kim J, Ziolkowski PA, Yelina NE, Hwang I, Martienssen RA, Henderson IR

Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.

Published on 10 Apr 2018 in Genome research , vol. 28 - pp 532-546

Choi K, Zhao X, Tock AJ, Lambing C, Underwood CJ, Hardcastle TJ , Serra H, Kim J, Cho HS, Kim J, Ziolkowski PA, Yelina NE, Hwang I, Martienssen RA, Henderson IR

Epigenetic activation of meiotic recombination near Arabidopsis thaliana centromeres via loss of H3K9me2 and non-CG DNA methylation.

Published on 10 Apr 2018 in Genome research , vol. 28 - pp 519-531

Underwood CJ, Choi K, Lambing C, Zhao X, Serra H, Borges F , Simorowski J, Ernst E, Jacob Y, Henderson IR, Martienssen RA

RAD51 and RTEL1 compensate telomere loss in the absence of telomerase.

Published on 16 Mar 2018 in Nucleic acids research , vol. 46 - pp 2432-2445

Olivier M , Charbonnel C , Amiard S , White CI , Gallego ME

Massive crossover elevation via combination of HEI10 and recq4a recq4b during Arabidopsis meiosis.

Published on 06 Mar 2018 in Proceedings of the National Academy of Sciences of the United States of America , vol. 115 - pp 2437-2442

Serra H , Lambing C, Griffin CH, Topp SD, Nageswaran DC, Underwood CJ, Ziolkowski PA, Séguéla-Arnaud M, Fernandes JB, Mercier R, Henderson IR

2017

Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis.

Published on 29 Aug 2017 in International journal of molecular sciences , vol. 18

Balc'h EL, Grandin N , Demattei MV, Guyétant S, Tallet A, Pagès JC, Ouaissi M, Lecomte T, Charbonneau M

RAD54 forms DNA repair foci in response to DNA damage in living plant cells.

Published on 30 Apr 2017 in The Plant journal : for cell and molecular biology , vol. 90 - pp 372-382

Hirakawa T, Hasegawa J, White CI , Matsunaga S

Quantification and Sequencing of Crossover Recombinant Molecules from Arabidopsis Pollen DNA.

Published on 31 Jan 2017 in Methods in molecular biology (Clifton, N.J.) , vol. 1551 - pp 23-57

Choi K, Yelina NE, Serra H , Henderson IR

CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the moss Physcomitrella patens.

Published on 30 Jan 2017 in Plant biotechnology journal , vol. 15 - pp 122-131

Collonnier C, Epert A, Mara K, Maclot F, Guyon-Debast A, Charlot F, White C , Schaefer DG, Nogué F

2016

Role of the Polymerase ϵ sub-unit DPB2 in DNA replication, cell cycle regulation and DNA damage response in Arabidopsis.

Published on 06 Sep 2016 in Nucleic acids research , vol. 44 - pp 7251-66

Pedroza-Garcia JA, Domenichini S, Mazubert C, Bourge M, White C , Hudik E, Bounon R, Tariq Z, Delannoy E, Del Olmo I, Piñeiro M, Jarillo JA, Bergounioux C, Benhamed M, Raynaud C

Recombination Rate Heterogeneity within Arabidopsis Disease Resistance Genes.

Published on 30 Jul 2016 in PLoS genetics , vol. 12 - pp e1006179

Choi K, Reinhard C, Serra H , Ziolkowski PA, Underwood CJ, Zhao X, Hardcastle TJ, Yelina NE, Griffin C, Jackson M, Mézard C, McVean G, Copenhaver GP, Henderson IR

The recombination mediator RAD51D promotes geminiviral infection.

Published on 30 Jun 2016 in Virology , vol. 493 - pp 113-27

Richter KS, Serra H , White CI , Jeske H

2015

Highly efficient radiosensitization of human glioblastoma and lung cancer cells by a G-quadruplex DNA binding compound.

Published on 06 Nov 2015 in Scientific reports , vol. 5 - pp 16255

Merle P, Gueugneau M, Teulade-Fichou MP, Müller-Barthélémy M, Amiard S , Chautard E, Guetta C, Dedieu V, Communal Y, Mergny JL, Gallego M , White C , Verrelle P, Tchirkov A

Centromere Associations in Meiotic Chromosome Pairing.

Published on 09 Sep 2015 in Annual review of genetics , vol. 49 - pp 95-114

Da Ines O , White CI

Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells.

Published on 30 Aug 2015 in Molecular and cellular biology , vol. 35 - pp 2818-30

Eid R, Demattei MV, Episkopou H, Augé-Gouillou C, Decottignies A, Grandin N , Charbonneau M

Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants.

Published on 30 Jan 2015 in The Plant journal : for cell and molecular biology , vol. 81 - pp 198-209

Muchová V, Amiard S , Mozgová I, Dvořáčková M, Gallego ME, White C , Fajkus J

2014

Arabidopsis thaliana RNase H2 deficiency counteracts the needs for the WEE1 checkpoint kinase but triggers genome instability.

Published on 30 Sep 2014 in The Plant cell , vol. 26 - pp 3680-92

Kalhorzadeh P, Hu Z, Cools T, Amiard S , Willing EM, De Winne N, Gevaert K, De Jaeger G, Schneeberger K, White CI , De Veylder L

Multiple host-cell recombination pathways act in Agrobacterium-mediated transformation of plant cells.

Published on 02 Mar 2014 in The Plant journal : for cell and molecular biology , vol. 77 - pp 511-20

Mestiri I , Norre F, Gallego ME , White CI

Responses to telomere erosion in plants.

Published on 01 Mar 2014 in PloS one , vol. 9 - pp e86220

Amiard S , Da Ines O , Gallego ME , White CI

2013

Signaling of double strand breaks and deprotected telomeres in Arabidopsis.

Published on 16 Oct 2013 in Frontiers in plant science , vol. 4 - pp 405

Amiard S , Gallego ME , White CI

Effects of XRCC2 and RAD51B mutations on somatic and meiotic recombination in Arabidopsis thaliana.

Published on 30 Jun 2013 in The Plant journal : for cell and molecular biology , vol. 74 - pp 959-70

Da Ines O , Degroote F , Amiard S , Goubely C , Gallego ME , White CI

Gene targeting in maize by somatic ectopic recombination.

Published on 30 Apr 2013 in Plant biotechnology journal , vol. 11 - pp 305-14

Ayar A, Wehrkamp-Richter S, Laffaire JB, Le Goff S , Levy J, Chaignon S, Salmi H, Lepicard A, Sallaud C, Gallego ME , White CI , Paul W

Regulation of Arabidopsis leaf hydraulics involves light-dependent phosphorylation of aquaporins in veins.

Published on 30 Mar 2013 in The Plant cell , vol. 25 - pp 1029-39

Prado K, Boursiac Y, Tournaire-Roux C, Monneuse JM, Postaire O, Da Ines O , Schäffner AR, Hem S, Santoni V, Maurel C

2012

Auxin regulates aquaporin function to facilitate lateral root emergence.

Published on 30 Oct 2012 in Nature cell biology , vol. 14 - pp 991-8

Péret B, Li G, Zhao J, Band LR, Voß U, Postaire O, Luu DT, Da Ines O , Casimiro I, Lucas M, Wells DM, Lazzerini L, Nacry P, King JR, Jensen OE, Schäffner AR, Maurel C, Bennett MJ

Gene Site-Specific Insertion in Plants.

Published on 21 Jun 2012 in Topics in Current Genetics, Springer, Dordrecht. , vol. 23

Olivier Da Ines & Charles I. White

A role for small RNAs in DNA double-strand break repair.

Published on 30 Mar 2012 in Cell , vol. 149 - pp 101-12

Wei W, Ba Z, Gao M, Wu Y, Ma Y, Amiard S , White CI , Rendtlew Danielsen JM, Yang YG, Qi Y

2011

Recombination proteins and telomere stability in plants.

Published on 30 Mar 2011 in Current protein & peptide science , vol. 12 - pp 84-92

Amiard S , White C , Gallego ME

Conserved CDC20 cell cycle functions are carried out by two of the five isoforms in Arabidopsis thaliana.

Published on 01 Jan 2011 in PloS one , vol. 6 - pp e20618

Kevei Z , Baloban M, Da Ines O , Tiricz H, Kroll A, Regulski K, Mergaert P, Kondorosi E

2010

Xrcc1-dependent and Ku-dependent DNA double-strand break repair kinetics in Arabidopsis plants.

Published on 30 Oct 2010 in The Plant journal : for cell and molecular biology , vol. 64 - pp 280-90

Charbonnel C , Gallego ME , White CI

Kinetic analyses of plant water relocation using deuterium as tracer – reduced water flux of Arabidopsis pip2 aquaporin knockout mutants.

Published on 30 Sep 2010 in Plant biology (Stuttgart, Germany) , vol. 12 Suppl 1 - pp 129-39

Da Ines O , Graf W, Franck KI, Albert A, Winkler JB, Scherb H, Stichler W, Schäffner AR

2009

APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root.

Published on 14 Jul 2009 in Proceedings of the National Academy of Sciences of the United States of America , vol. 106 - pp 11806-11

Vanstraelen M, Baloban M, Da Ines O , Cultrone A, Lammens T, Boudolf V, Brown SC, De Veylder L, Mergaert P, Kondorosi E

ERCC1/XPF protects short telomeres from homologous recombination in Arabidopsis thaliana.

Published on 02 Mar 2009 in PLoS genetics , vol. 5 - pp e1000380

Vannier JB, Depeiges A , White C , Gallego ME

Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance.

Published on 01 Mar 2009 in DNA repair , vol. 8 - pp 413-9

Kozak J, West CE, White C , da Costa-Nunes JA, Angelis KJ

2008

Theses

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