Michel CHARBONNEAU
Directeur de Recherche
CHARBONNEAU
Michel
Directeur de Recherche
DR
Resume
Major stages of career:
- DEA de Biologie Cellulaire et Moléculaire, Septembre 1978, Rennes (Bien).
- Thèse de 3ème cycle, speciality : Biologie Cellulaire et Moléculaire, Novembre 1981, Rennes (Très Honorable).
- 1st post-doc, University of California, Davis, USA, 1981-1983.
- Recruitment at CNRS, Octobre 1982.
- Thèse d'Etat ès-Sciences Naturelles, Décembre 1985, Rennes (Très Honorable).
- 2ndpost-doc, Scripps Research Institute, La Jolla, California, USA, 1992-1994.
Affectations:
- URA CNRS 256, University of Rennes I, 1977-1992 (avec interruptions).
- Station Biologique de Roscoff (LP CNRS 4601), 1978-1981.
- Department of Zoology, University of California, Davis, USA, 1981-1982.
- Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, USA, 1992-1994.
- Ecole Normale Supérieure de Lyon, UMR CNRS/ENS 5239 (LBMC), Lyon, 1994-2010.
- UMR CNRS 7292 (GICC)/University of Tours, 2010-2017.
- UMR CNRS 6293/INSERM U1103-iGReD, Clermont-Ferrand, 2017.
Research
* We have produced detailed scanning and transmission electron microscopy images analyzing the different stages of sperm-egg interactions in Anuran and Urodela amphibians (PMID: 6983590).
* Thanks to electrophysiology techniques, we were the first to establish that the natural polyspermic fertilization of Urodela amphibians (newts, salamanders) was due to the absence of electrical blocking against polyspermy, unlike what happens inAnurans (frogs and toads) with monospermic fertilization (PMID: 6603379).
* We found that the cortical endoplasmic reticulum of the Xenopus oocyte develops during its maturation in coincidence with the ability of the egg to propagate the intracellular Ca2+ wave generated during sperm activation (PMID: 6421640).
* We were the first to measure intracellular Ca2+ oscillations in embryos (using the amphibian, xenopus model) and to better characterize intracellular pH oscillations occurring simultaneously (PMID: 1993738 and PMID: 2116420).
* We have discovered, in the Saccharomyces cerevisiae model, two new telomeric proteins, Stn1 and Ten1, which together with Cdc13 (the main telomeric protein whose functions we have also well documented) form the conserved telomeric complex CST (PMID: 9042864, PMID:11230140 and PMID: 11046137).
* We challenged the dogma that telomere senescence resulted solely from telomere shortening caused by telomerase inactivation by showing that loss of telomere protection in Cdc13 mutants also caused this senescence in the presence of functional telomerase, followed byof the production of post-senescence survivors maintaining functional telomeres by recombination (instead of telomerase) (PMID: 11689452).
* We found that the DNA replication checkpoint protein, Mrc1, in cooperation with the kinase Rad53, was activated during senescence caused by telomerase inactivation, while senescence caused by the losstelomeric protection (Cdc13 and Ku) in the presence of functional telomerase activated Rad53-Rad9 but not Rad53-Mrc1 (PMID: 15760303).
* We have identified a third maintenance pathway for functional telomeres (in addition to the telomerase pathway and the ALT pathway (recombination-dependent, telomerase-independent) which we have called the ILT (“Inherited Long Telomere”) pathway (PMID: 19047370).
* We have initiated or co-initiated clinical studies consisting in evaluating the importance of telomeric parameters in several types of cancer (PMID: 28850092, PMID: 28755323, PMID: 31706351 and PMID: 34690007).
* We have discovered a new function of the Cdc13-Stn1-Ten1 telomeric complex, which consists in the regulation of the elongation phase of transcription by RNA polymerase II (PMID: 31006804).
Publications
The TeloDIAG: how telomeric parameters can help in glioma rapid diagnosis and liquid biopsy approaches.
Published on 30 Dec 2021 in Annals of oncology : official journal of the European Society for Medical Oncology , vol. 32 - pp 1608-1617
Billard P, Guerriau C, Carpentier C, Juillard F, Grandin N , Lomonte P, Kantapareddy P, Dufay N, Barritault M, Rimokh R, Verrelle P, Maucort-Boulch D, Figarella-Branger D, Ducray F, Dehais C, Charbonneau M , Meyronet D, Poncet DA, POLA network
Inhibition of the alternative lengthening of telomeres pathway by subtelomeric sequences in Saccharomyces cerevisiae.
Published on 30 Dec 2020 in DNA repair , vol. 96 - pp 102996
The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription.
Published on 09 Jul 2019 in Nucleic acids research , vol. 47 - pp 6250-6268
Calvo O, Grandin N , Jordán-Pla A, Miñambres E, González-Polo N, Pérez-Ortín JE, Charbonneau M
The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas.
Published on 09 Nov 2019 in Acta neuropathologica communications , vol. 7 - pp 175
Grandin N , Pereira B, Cohen C, Billard P, Dehais C, Carpentier C, Idbaih A, Bielle F, Ducray F, Figarella-Branger D, Delattre JY, Sanson M, Lomonte P, Poncet D, Verrelle P, Charbonneau M , POLA network
Measurement of Telomere Length in Colorectal Cancers for Improved Molecular Diagnosis.
Published on 29 Aug 2017 in International journal of molecular sciences , vol. 18
Balc'h EL, Grandin N , Demattei MV, Guyétant S, Tallet A, Pagès JC, Ouaissi M, Lecomte T, Charbonneau M
Detection of the alternative lengthening of telomeres pathway in malignant gliomas for improved molecular diagnosis.
Published on 30 Nov 2017 in Journal of neuro-oncology , vol. 135 - pp 381-390
Fogli A , Demattei MV, Corset L, Vaurs-Barrière C , Chautard E, Biau J, Kémény JL, Godfraind C, Pereira B, Khalil T, Grandin N , Arnaud P , Charbonneau M , Verrelle P
Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells.
Published on 30 Aug 2015 in Molecular and cellular biology , vol. 35 - pp 2818-30
Eid R, Demattei MV, Episkopou H, Augé-Gouillou C, Decottignies A, Grandin N , Charbonneau M
