Platform staff are responsible for advising, supporting and training users in the autonomous use of the equipment.

The CLIC team organizes meetings to ensure project feasibility, to define experimental conditions (fluorophores, type of set-up, controls) and equipment to be used (autonomy after training or support. These meetings also allow to anticipate image analysis requirements, so that CLIC can define the most appropriate imaging set-ups and analysis method and train the user if needed.

At the beginning of the project, a test session on samples is proposed to determine the best microscope and optimal acquisition conditions.

During the project, follow-up meetings are proposed as user needs may evolve. OMERO, a data management solution, is proposed to users to store their image data, either internally for iGReD users or at the Auvergne Bio-informatique (AuBi) Platform for external users. 

CLIC offers a wide range of microscopes with different acquisition modes to improve speed, sensitivity and spatial or spectral resolution :

–  Zeiss LSM 800 AiryScan

– Zeiss LSM 980 Airyscan 2 + spectral detection module

–  Zeiss Cell Observer Spinning Disk

–  Leica TCS SP5 Resonant Scanner

– Leica TCS SP8 Lightning Scanner Resonant Navigator

–  Leica MMAF

– ZEISS Lightsheet LS7

To complement the use of the microscopes, the platform provides 3 image  analysis stations equipped with a wide range of specialized software (Fiji, Icy, Qupath, Zen… , ARIVIS, 2 IMARIS licenses).