The Clermont confocal imaging platform (CLIC) is an essential ressource for biology and health researchers in Clermont-Ferrand. The platform CLIC offers several confocal microscopes and image analysis stations within the Institute of Genetics, Reproduction and Development (iGReD), which is hosted by the University Clermont Auvergne (UCA) and supported by CNRS and INSERM. It is one of 5 platforms in the Microscopy-Imaging cluster of the UCA-Partner university's general service.
The range of biological samples processed is vast and includes bacteria, biofilms, viruses, plants, insects, mammals and specific materials such as polymers. Samples can be analysed in different states - living or fixed, tissues or organs, sections, organoids or cell cultures.
Funders
Missions
Platform staff are responsible for advising, supporting and training users in the autonomous use of the equipment.
The CLIC team organizes meetings to ensure project feasibility, to define experimental conditions (fluorophores, type of set-up, controls) and equipment to be used (autonomy after training or support. These meetings also allow to anticipate image analysis requirements, so that CLIC can define the most appropriate imaging set-ups and analysis method and train the user if needed.
At the beginning of the project, a test session on samples is proposed to determine the best microscope and optimal acquisition conditions.
During the project, follow-up meetings are proposed as user needs may evolve. OMERO, a data management solution, is proposed to users to store their image data, either internally for iGReD users or at the Auvergne Bio-informatique (AuBi) Platform for external users.
CLIC offers a wide range of microscopes with different acquisition modes to improve speed, sensitivity and spatial or spectral resolution :
– Zeiss LSM 800 AiryScan
– Zeiss LSM 980 Airyscan 2 + spectral detection module
– Zeiss Cell Observer Spinning Disk
– Leica TCS SP5 Resonant Scanner
– Leica TCS SP8 Lightning Scanner Resonant Navigator
– Leica MMAF
To complement the use of the microscopes, the platform provides 3 image analysis stations equipped with a wide range of specialized software (Fiji, Icy, Qupath, Zen… plus 2 IMARIS licenses).
Access conditions
Scientifics Directors
Technical director
Members
Publication
Automated 3D bio-imaging analysis of nuclear organization by NucleusJ 2.0.
Published on 30 Dec 2020 in Nucleus (Austin, Tex.) , vol. 11 - pp 315-329
Dubos T , Poulet A , Gonthier-Gueret C, Mougeot G , Vanrobays E , Li Y , Tutois S , Pery E, Chausse F, Probst AV, Tatout C , Desset S
