Using the plant Arabidopsis thaliana as a model organism, our team studies how these different aspects of chromatin organization allow an adequate transcriptional response during development or in response to environmental stimuli.
To better understand the contribution of these epigenetic mechanisms in transcriptional regulation, we characterize the composition of nucleosomes in histone variants and study how these histone variants are stored, transported and incorporated into chromatin by histone chaperones (Le Goff et al, 2019, Probst, 2022). We further investigate the influence of chromatin structure on transcription via the study of GH1-domain chromatin proteins that include linker histone H1 and non-histone proteins of the TRB and GH1-HMGA type (Charbonnel et al, 2018, Teano et al, 2022). Finally, our studies of the interactions between chromatin, lamina and the nuclear envelope have led us to better understand the relationship between chromatin position in nuclear space and transcriptional activity (Poulet et al, 2017, Mermet et al, 2021).
We use molecular biology, genetics, epigenetics and bioinformatics approaches and develop new bio-imaging tools including artificial intelligence to monitor chromatin dynamics imaged by confocal microscopy (Dubos et al, 2020, 2022, Mougeot et al, 2022).
Our team is composed of two CNRS researchers, an INSERM engineer, six Associate Professors from the Clermont Auvergne University and several Master and PhD students. Our research is integrated into local networks including the CAP20-25 project International research Centre 1 (CIR1) on Sustainable Agro-ecosystems and CIR3 on mobility, national networks such as the GDR EPIPLANT, the GDR ADN&G, the GDR IMABIO, or the RtmFM network, European networks such as the Marie Curie Doctoral Network EpiSeedLink and international networks through our involvement in the Society of Experimental Biology (SEB).
We are strongly involved in the life of Clermont Auvergne University through our implication in the UFR Biology and our contribution to courses in genetics in the Bachelor of Life Sciences, epigenetics in the Master of Health Biology and in the Master of Plant Biology, and bioinformatics in the Master of Bioinformatics. We also contribute to dissemination of scientific culture through our participation in the astu’science association.
Evolutionarily conserved protein motifs drive interactions between the plant nucleoskeleton and nuclear pores
Published on 21 Sep 2023 in The Plant Cell
Published on 28 Jul 2023 in Cell Reports , vol. 42 - pp 112894
Gianluca Teano, Lorenzo Concia, Léa Wolff, Léopold Carron, Ivona Biocanin, Kateřina Adamusová, Miloslava Fojtová, Michael Bourge, Amira Kramdi, Vincent Colot, Ueli Grossniklaus, Chris Bowler, Célia Baroux, Alessandra Carbone, Aline V Probst , Petra Procházková Schrumpfová, Jiří Fajkus, Simon Amiard , Stefan Grob, Clara Bourbousse, Fredy Barneche
Published on 26 Jan 2023 in The Plant journal : for cell and molecular biology
Published on 30 Dec 2022 in Nucleus (Austin, Tex.) , vol. 13 - pp 277-299
Randall RS, Jourdain C, Nowicka A, Kaduchová K, Kubová M, Ayoub MA, Schubert V, Tatout C , Colas I, Kalyanikrishna, Desset S , Mermet S , Boulaflous-Stevens A, Kubalová I, Mandáková T, Heckmann S, Lysak MA, Panatta M, Santoro R, Schubert D, Pecinka A, Routh D, Baroux C
Published on 06 Dec 2022 in bioRxiv
Gianluca Teano, Lorenzo Concia, Léa Wolff, Léopold Carron, Ivona Biocanin, Kateřina Adamusová, Miloslava Fojtová, Michael Bourge, Amira Kramdi, Vincent Colot, Ueli Grossniklaus, Chris Bowler, Célia Baroux, Alessandra Carbone, Aline V. Probst , Petra Procházková Schrumpfová, Jiří Fajkus, Simon Amiard , Stefan Grob, Clara Bourbousse, Fredy Barneche
Published on 24 Nov 2022 in F1000Research , vol. 11 - pp 392
Published on 01 Apr 2022 in Journal of cell science , vol. 135
Published on 06 Jul 2021 in Frontiers in plant science , vol. 12 - pp 677849
Meschichi A, Ingouff M, Picart C, Mirouze M, Desset S , Gallardo F, Bystricky K, Picault N, Rosa S, Pontvianne F
Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis.
Published on 03 Jun 2021 in Genome research , vol. 31 - pp 1230-44
Huang Y, Sicar S, Ramirez-Prado JS, Manza-Mianza D, Antunez-Sanchez J, Brik-Chaouche R, Rodriguez-Granados NY, An J, Bergounioux C, Mahfouz MM, Hirt H, Crespi M, Concia L, Barneche F, Amiard S , Probst AV , Gutierrez-Marcos J, Ariel F, Raynaud C, Latrasse D, Benhamed M
Published on 30 Jan 2020 in The Plant journal : for cell and molecular biology , vol. 101 - pp 71-86
Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions.
Published on 30 Dec 2019 in Nucleus (Austin, Tex.) , vol. 10 - pp 181-212
Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development.
Published on 30 Jan 2019 in The New phytologist , vol. 221 - pp 385-398
Published on 30 Jun 2018 in Chromosoma , vol. 127 - pp 247-259
Kemeny S, Tatout C , Salaun G, Pebrel-Richard C, Goumy C, Ollier N, Maurin E, Pereira B, Vago P, Gouas L
Published on 30 May 2018 in Plant physiology , vol. 177 - pp 311-327
Genetic and epigenetic variation in 5S ribosomal RNA genes reveals genome dynamics in Arabidopsis thaliana.
Published on 06 Apr 2018 in Nucleic acids research , vol. 46 - pp 3019-3033
High-Affinity LNA-DNA Mixmer Probes for Detection of Chromosome-Specific Polymorphisms of 5S rDNA Repeats in Arabidopsis thaliana.
Published on 01 Jan 2018 in Methods in molecular biology (Clifton, N.J.) , vol. 1675 - pp 481-491
Simon L , Probst AV
Quantitative 3D Analysis of Nuclear Morphology and Heterochromatin Organization from Whole-Mount Plant Tissue Using NucleusJ.
Published on 01 Jan 2018 in Methods in molecular biology (Clifton, N.J.) , vol. 1675 - pp 615-632
Published on 01 Jan 2018 in Methods in molecular biology (Clifton, N.J.) , vol. 1840 - pp 205-219
Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System.
Published on 01 Jan 2018 in Methods in molecular biology (Clifton, N.J.) , vol. 1840 - pp 221-235
Published on 02 Jan 2017 in Nucleus (Austin, Tex.) , vol. 8 - pp 46-59
Published on 30 Oct 2016 in Journal of experimental botany , vol. 67 - pp 5699-5710
Published on 01 Apr 2015 in Bioinformatics (Oxford, England) , vol. 31 - pp 1144-6
The histone chaperone complex HIR maintains nucleosome occupancy and counterbalances impaired histone deposition in CAF-1 complex mutants.
Published on 30 Mar 2015 in The Plant journal : for cell and molecular biology , vol. 81 - pp 707-22
Published on 01 Jan 2015 in Nucleus (Austin, Tex.) , vol. 6 - pp 471-8
Characterization of two distinct subfamilies of SUN-domain proteins in Arabidopsis and their interactions with the novel KASH-domain protein AtTIK.
Published on 30 Dec 2014 in Journal of experimental botany , vol. 65 - pp 6499-512
Published on 23 Oct 2014 in BMC genomics , vol. 15 - pp 922
Transcript levels, alternative splicing and proteolytic cleavage of TFIIIA control 5S rRNA accumulation during Arabidopsis thaliana development.
Published on 30 Jul 2012 in The Plant journal : for cell and molecular biology , vol. 71 - pp 35-44
Published on 30 Mar 2012 in Nucleic acids research , vol. 40 - pp 2566-76
Characterization of a cinnamoyl-CoA reductase 1 (CCR1) mutant in maize: effects on lignification, fibre development, and global gene expression.
Published on 30 Jul 2011 in Journal of experimental botany , vol. 62 - pp 3837-48
Tamasloukht B, Wong Quai Lam MS, Martinez Y, Tozo K, Barbier O, Jourda C, Jauneau A, Borderies G, Balzergue S, Renou JP, Huguet S, Martinant JP, Tatout C , Lapierre C, Barrière Y, Goffner D, Pichon M
TRF2 and apollo cooperate with topoisomerase 2alpha to protect human telomeres from replicative damage.
Published on 23 Jul 2010 in Cell , vol. 142 - pp 230-42
Ye J, Lenain C, Bauwens S, Rizzo A, Saint-Léger A, Poulet A , Benarroch D, Magdinier F, Morere J, Amiard S , Verhoeyen E, Britton S, Calsou P, Salles B, Bizard A, Nadal M, Salvati E, Sabatier L, Wu Y, Biroccio A, Londoño-Vallejo A, Giraud-Panis MJ, Gilson E
Published on 30 Jun 2010 in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry , vol. 15 - pp 641-54
Published on 30 Jan 2010 in BioTechniques , vol. 48 - pp 53-60
Vanrobays E , Jennings BH, Ish-Horowicz D
Published on 30 Oct 2009 in PLoS genetics , vol. 5 - pp e1000690
Published on 18 Mar 2009 in The EMBO journal , vol. 28 - pp 641-51
Poulet A , Buisson R, Faivre-Moskalenko C, Koelblen M, Amiard S , Montel F, Cuesta-Lopez S, Bornet O, Guerlesquin F, Godet T, Moukhtar J, Argoul F, Déclais AC, Lilley DM, Ip SC, West SC, Gilson E, Giraud-Panis MJ
TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export.
Published on 30 Oct 2008 in RNA (New York, N.Y.) , vol. 14 - pp 2061-73
Vanrobays E , Leplus A, Osheim YN, Beyer AL, Wacheul L, Lafontaine DL
Published on 30 Apr 2008 in The Plant journal : for cell and molecular biology , vol. 54 - pp 299-309
In Drosophila melanogaster the COM locus directs the somatic silencing of two retrotransposons through both Piwi-dependent and -independent pathways.
Published on 06 Feb 2008 in PloS one , vol. 3 - pp e1526